ERAP1 Knockout cell line (Hep G2)

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ERAP1 Knockout cell line (Hep G2) Order

ERAP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT5174
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ERAP1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ERAP1 Knockout cell line (Hep G2)
Species
Human
Gene Name
ERAP1
Gene ID
51752
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript ERAP1-202)
Validation: PCR+Sanger Sequencing
ERAP1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ERAP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ERAP1 gene knockout ERAP1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ERAP1 Knockout cell line (Hep G2)
ERAP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT5174
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ERAP1 Knockout cell line (HeLa)

    ERAP1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88005
    YKO-XN88005(HeLa)
  • ERAP1 Knockout cell line (HCT 116)

    ERAP1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88004
    YKO-XN88004(HCT 116)
  • ERAP1 Knockout cell line (HEK293)

    ERAP1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88003
    YKO-XN88003(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ERAP1 Knockout cell line (HeLa)

    ERAP1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88005
      inquiry   More details
  • ERAP1 Knockout cell line (HCT 116)

    ERAP1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88004
      inquiry   More details
  • ERAP1 Knockout cell line (HEK293)

    ERAP1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88003
      inquiry   More details

ERAP1 Knockout cell line (Hep G2) Order

ERAP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT5174
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ERAP1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ERAP1 Knockout cell line (Hep G2)
Species
Human
Gene Name
ERAP1
Gene ID
51752
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript ERAP1-202)
Validation: PCR+Sanger Sequencing
ERAP1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ERAP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ERAP1 gene knockout ERAP1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ERAP1 Knockout cell line (Hep G2)
ERAP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT5174
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ERAP1 Knockout cell line (HeLa)

    ERAP1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88005
    YKO-XN88005(HeLa)
  • ERAP1 Knockout cell line (HCT 116)

    ERAP1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88004
    YKO-XN88004(HCT 116)
  • ERAP1 Knockout cell line (HEK293)

    ERAP1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88003
    YKO-XN88003(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ERAP1 Knockout cell line (HeLa)

    ERAP1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88005
      inquiry   More details
  • ERAP1 Knockout cell line (HCT 116)

    ERAP1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88004
      inquiry   More details
  • ERAP1 Knockout cell line (HEK293)

    ERAP1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ERAP1
    Gene ID: 51752
    Size: 1*10^6
    Catalog#: YKO-XN88003
      inquiry   More details

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